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KMID : 0613820060160071158
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Journal of Life Science
2006 Volume.16 No. 7 p.1158 ~ p.1163
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Isolation of Acinetobacter calcoaceticus BP-2 Capable of Degradation of Bisphenol A
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Kwon Gi-Seok
Kim Dong-Geol
Lee Jung-Bok
Shin Kee-Sun
Kum Eun-Joo
Sohn Ho-Yong
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Abstract
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Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl)propane, has been widely used as a monomer for production of epoxy resins and polycarbonate plastics, and final products of BPA include adhesives, protective coatings, paints, optical lens, building materials, compact disks and other electrical parts. Since BPA is a toxic chemical to elicit acute cell cytotoxicity and chronic endocrine disrupting activity, the degradation of BPA has been focused during last decades. To overcome the problem of photo-, and chemical-degradation of BPA, in this study, a bacterium that is able to biodegrade BPA, was isolated. The bacterium, isolated from the soil of plastic factory, was identified as Acinetobacter calcoaceticus (strain BP-2) based on physiological and 16S rDNA sequencing analysis. A. calcoaceticus BP-2 was able to grow in the presence of 1140 μg ml-1 BPA. Biodegradation experiments showed that BP-2 mineralized BPA via 4-hydroxybenzoic acid and 4-hydroxyacetophenone, and average degradation rate was 53.3 μg ml-1 day-1 under optimal conditions (pH 7 and 30℃). In high density resting cell (3.5 g-dcw.l-1) experiments, the maximal degradation rate was increased to 89.7 μg ml-1 h-1. Our results suggest that BP-2 has high potential as a catalyst for practical BPA bioremediation.
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KEYWORD
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Acinetobacter calcoaceticus BP-2, biodegradation, bisphenol A, detoxification
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